Please help! D1 (2 points). How much agarose do you need to make 75 ml of a 1% a
ID: 34021 • Letter: P
Question
Please help!
D1 (2 points). How much agarose do you need to make 75 ml of a 1% agarose gel? Show your wart Before proceeding to the next step, your calculations must be checked by an instructor. D2 (3 points). Use the 1 kl3 ladder on your gel to determine the approximate fragment sizes from your two digestions. Record the approximate sizes here. The molecular weight marker we used is shown below. What does the digestion pattern indicate about how many times each restriction enzyme cut the plasmid? Base PairsExplanation / Answer
D1) Agarose is a gelling agent that polymerizes the gel required for running gel electrophoresis.
To prepare 75 ml of 1% agarose gel, 0.75 gms of agarose is required.
D2) The gel indicates that restriction enzyme has cut the plamid at 2 places, thereby, giving 2 fragments with varying molecular weights