Consider the mutations described in the left-hand column of the following table.

Question

Consider the mutations described in the left-hand column of the following table. Indicate whether the lac operon will be on or off in each of the four different environments listed across the top. No explanation needed.

Mutation

No lactose or glucose

Lactose only

Lactose and Glucose

Glucose only

Wild type operon

CRP cannot bind promoter

CRP cannot bind cAMP

Lac repressor lost lactose binding activity

Lac repressor cannot bind operator

Mutation

No lactose or glucose

Lactose only

Lactose and Glucose

Glucose only

Wild type operon

CRP cannot bind promoter

CRP cannot bind cAMP

Lac repressor lost lactose binding activity

Lac repressor cannot bind operator

Explanation / Answer

Mutation

Mutation

No lactose or glucose Lactose only Lactose and Glucose Glucose only Wild type operon off On On off CRP cannot bind promoter off off On off CRP cannot bind cAMP off off off off Lac repressor lost lactose binding activity off off off off Lac repressor cannot bind operator On On On On

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