Calclulate the extinction coefficient (in units of M -1 cm -1 ) for bromophenol
ID: 475777 • Letter: C
Question
Calclulate the extinction coefficient (in units of M-1 cm-1) for bromophenol blue at lambda max using Beer's law (A=ebc). b=1 cm.
(0.1 M bromophenol blue used)
Use A409 to calculatethe concentration of myoglobin in the inidial stock solution that is made by diluting the myoglobin powder. (5 mg of myoglobin powder in 1 mL phosphate buffer).
2. Calculate the extinction coefficient (in units of MT cm 1) for bromophenol blue at nmax using Beer's law (A ebc). Conveniently for you, your cuvettes had a 1 cm path length. where: € extinction coefficient in M-1 cm 400 if max 400 nm, 400 absorption bc b cuvette path length in cm c -concentration in moles/L 3. Use A409 to calculate the concentration of myoglobin in the initial stock solution that you made by diluting the myoglobin powder.Explanation / Answer
Answer 2. As the absorbance value is not mentioned, (looks like it has to be detrmined via experimental means or lab), I assume the value to be somewhere around 1. So, extinction coefficient will be e = 1/ (0.1 M x 1cm)= 10 M-1cm-1.
So, by using correct A values, correct e can be determined.