In the enzyme kinetics experiment, you obtain the following results, using an ex
ID: 67712 • Letter: I
Question
In the enzyme kinetics experiment, you obtain the following results, using an excess of pyruvate: Lactate dehydrogenase is a tetrameric enzyme. Based on the data above, is there any evidence of cooperative substrate binding to this tetramer? Determine the value of Vmax and of the Hill coefficient based on the data above. Provide an equation that relates the fractional saturation of the enzyme (Y = enzyme bound to substrate / total enzyme) to Vmax and to the initial reaction rate. Based on your experimental data, generate a Hill plot for the enzyme you produced. What does the slope of this plot represent?Explanation / Answer
Fractional saturation rate = Vo/V max
Hill coefficient =