Please answer all the questions regarding the case study/scenario below. Thank y
ID: 84496 • Letter: P
Question
Please answer all the questions regarding the case study/scenario below. Thank you!You are involved in a research team working on how the mutation affects the function of the genome. The research group is now focusing on how the mutations on the upstream of the open reading frame affect on the expression of a specific gene.
(1) Generate a hypothesis on the potential effect of mutations on how the mutation on the upstream of a gene can alter the gene expression.
(2) Design experimental strategy of mutational analysis and describe any anticipated result (predicted outcome from your experimental design). Please answer all the questions regarding the case study/scenario below. Thank you!
You are involved in a research team working on how the mutation affects the function of the genome. The research group is now focusing on how the mutations on the upstream of the open reading frame affect on the expression of a specific gene.
(1) Generate a hypothesis on the potential effect of mutations on how the mutation on the upstream of a gene can alter the gene expression.
(2) Design experimental strategy of mutational analysis and describe any anticipated result (predicted outcome from your experimental design). Please answer all the questions regarding the case study/scenario below. Thank you!
You are involved in a research team working on how the mutation affects the function of the genome. The research group is now focusing on how the mutations on the upstream of the open reading frame affect on the expression of a specific gene.
(1) Generate a hypothesis on the potential effect of mutations on how the mutation on the upstream of a gene can alter the gene expression.
(2) Design experimental strategy of mutational analysis and describe any anticipated result (predicted outcome from your experimental design).
Explanation / Answer
Hi,
The mutations can occur anywhere in the genome. In the first scenario, we need to analyze what happens to the expression of the gene when there are mutations upstream of it.
The mutational analysis can be carried out using targeted mutagenesis.