Please answer all the questions regarding the case study/scenario below. Thank y
ID: 84565 • Letter: P
Question
Please answer all the questions regarding the case study/scenario below. Thank you! You are involved in a research team working on how the mutation affects the function of the genome. The research group is now focusing on how the mutations on the upstream of the open reading frame affect on the expression of a specific gene.
(1) Generate a hypothesis on the potential effect of mutations on how the mutation on the upstream of a gene can alter the gene expression.
(2) Design experimental strategy of mutational analysis and describe any anticipated result (predicted outcome from your experimental design).
Explanation / Answer
(1) A hypothesis on the potential effects can be:
Promoter elements are present in the upstream of gene which are essential for gene expression. There may be some regulatory regions present also. Mutation in upstream gene may cause impairment of functions of the regulatory regions and cause a reuction in gene expression levels. Or the gene may also get silenced.
(2) The anticipated results are described as follows:
In case there is mutation in promoter region then polymerase will not be able to bind the promoter.
In case there is mutation in operator then activators will not be able to bind and gene will not be expressed.