Map pling The table below shows the data for purification of a hypothetical prot
ID: 875448 • Letter: M
Question
Map pling The table below shows the data for purification of a hypothetical protein. Complete the table by filling in the missing values for the specific activity, yield, and purification level tal Protein Total Activity Specific Activity Yield Purification Step (mg) (units) (units mg level 153000 Homogenization 17700 8.64 100 5040 Salt fractionation 138000 89.9 3.17 27.4 Ion-exchange chromatography 1.50 x 103 117000 Number Number Number Gel-filtration chromatography 79700 85.1 Affinity 55800 chromatography 4.27 Note: A red X will appear in the upper left corner of any box that has an incorrect answer. If a box does not have a red Xat the upper left, that particular answer is correct.Explanation / Answer
Specific activity = total activity / total protein
% yield = (new activity / initial activity)*100
Purification level = specific activity calculated after each purification step / specific activity of the initial step
For Ion-exchange chromatography:-
Specific activity = 117000/1.50X103
= 78 units/mg
% yield = 117000/153000*100
= 76.47%
Purification level = 78/8.64
= 9.02
For Gel-Filtration Chromatography :-
Specific activity = 79700/85.1
= 936.54 units/mg
% yield = 79700/153000*100
= 52.09%
Purification level = 936.54/8.64
= 108.39
For Affinity Chromatography :-
Specific activity = 55800/4.27
= 13067.91 units/mg
% yield = 55800/153000*100
= 36.47
Purification level = 13067.91/8.64
= 1512.48