An enzyme-catalyzed reaction is carried out under conditions in which the substr
ID: 923035 • Letter: A
Question
An enzyme-catalyzed reaction is carried out under conditions in which the substrate concentration [S] is much higher than the Michaelis constant KM. Under these conditions, which of the following processes do you expect to lead to the greatest increase in the maximal reaction rate (Vmax) for the reaction: i) Increase the total enzyme concentration ii) increase kcat iii) increase KM iv) decrease KM.
i and ii
i and iii
i and iv
ii and iii
ii and iv
A.i and ii
B.i and iii
C.i and iv
D.ii and iii
E.ii and iv
Explanation / Answer
when Vmax is increasing the the total enzyme concentration increses and Kcat also increases because Vmax is directly proportional to Kcat and [E0] so answer is A) i and ii