Substance Data Table 1 lists 5 substances that will be tested for lipids. Predic
ID: 99309 • Letter: S
Question
Substance
Data Table 1 lists 5 substances that will be tested for lipids. Predict whether or not you think each substance will contain lipids, and record your predictions in Data Table 1.
Place the aluminum foil on a flat surface and place one piece of round filter paper on top of the aluminum foil.
Use the graduated pipet to draw up Sudan III. You must use the graduated pipet as seen in Figure 3, not a short stem pipet. Additionally, Sudan III will stain clothing, so be careful.
Place 1 drop of Sudan III in 5 places on the filter paper, as shown in Figure 4.
Allow each of the drops to completely dry before moving on to the next step. This will take less than 5 minutes.
Use a pencil to trace an outline around each of the dried Sudan III drops. You must use a pencil, as ink interferes with results.
Using the pencil, label each of the Sudan III drops for the substances listed in Data Table 1: dH2O, oil, egg, mayo, and C-M (for liquid coffee creamer).
Prepare a mayonnaise sample.
Use the marking pencil and a ruler to measure a line that is ½ cm from the bottom of the test tube.
Place a pea-sized portion of mayonnaise in the test tube. It is okay if the mayonnaise sticks to the side of the test tube.
Label a short-stem pipet "DW" and use it to add distilled water to the mayonnaise. Fill the test tube to the ½ cm mark. Place a gloved finger over the top of the test tube and shake to mix.
Prepare the egg white sample:
Crack a raw egg on the side of a bowl. Allow the egg white to flow from the cracked shell to the bowl.
Dispose of the yolk and shell in a trash bin.
To the egg white, use the graduated cylinder to add 25 mL of distilled water. Beat the egg white with a fork or whisk until completely mixed. The solution should be somewhat frothy.
Use a clean, short-stem pipet to add 1 drop of each substance to the center of its corresponding circle. Do not use a pipet for more than one substance.
Place the filter paper out of reach of small children and pets and allow the setup to rest for 1 hour.
After 1 hour, observe the filter paper to see which solutions have spread outside of the pencil markings. For each sample, use the ruler to measure the distance the Sudan III has traveled from the pencil marking to the edge of the solution, in millimeters. Record in Data Table 1.
A positive test for lipids is indicated if the red coloration of the Sudan III is carried outside of the pencil line. This indicates that the Sudan III is solubilized by the sample. The higher the concentration of lipids in the sample, the further the Sudan III will travel.
Allow the setup to rest overnight (24 hours total). After 24 hours, repeat Step 12.
In Data Table 1, record whether each substance tested positive or negative for lipids.
Substance
Lipid Prediction (Y or N) Distance Sudan III Traveled Outside of Circle after 1 hr (mm) Distance Sudan III Traveled Outside of Circle after 24 hrs (mm) Test Results for Lipids (positive or negative) Distilled Water Oil Beaten Egg White Mayonnaise Liquid Coffee CreamerExplanation / Answer
Ans- The distance travelled by the dye will be seen by performing the experiment but the prediction result will be as follow-up
Substance distilled water oil Beaten egg white myannoise Liquid coffee creamer Lipids prediction (y or n) n y y y y Test for lipid prediction (positive or negative) negative positive positive positive positive