Please check answers and show your work. Triosephosphate isomerase (TIM) is an e
ID: 210834 • Letter: P
Question
Please check answers and show your work.
Triosephosphate isomerase (TIM) is an enzyme that functions in the conversion of dihydroxyacetone phosphate to glyceraldehydes-3-phosphate during Glycolysis. A associated with recurrent infections, neurological disorders, and death in some individuals. In some affected individuals, the TIM deficiency is caused by a mutation that changes a phenyalanine codon (TTC) to a leucine codon (CTC), which results in a thermolabile TIM protein ( tempera true or false. the protein does not function at elevated d on this information, tell whether the following statements are a. The mutation that creates a non-functioning TIM protein is a nonsense rb. The TIM deficiency mutation changes the tertiary structure of the protein mutation such that the new tertiary structure does not function at high temperatures.. In the above case, the TIM deficiency does not result from a base substitution. c. shown below is a DNA molecule that is part of the RNA-coding sequence of a transcriptional unit or gene. The top strand is the template strand. Respond to the following questions (think and analyze carefully) TEMPLATE 5' GCAAATGGCATGTA 3' 3 CGTITTACCGTACAT 5 A) GAA G Give the mRNA sequence of the transcribed gene. Label the 5' and 3' ends of the mRNA What is the amino acid sequence of the protein gene product? Use the codon table provided. Assume the first codon is located at the extreme 5' end of your mRNA sequence. Label the amino terminal end and the carboxy terminal end of the protein. a. b.Explanation / Answer
a. The mutation that creates a non functioning Tim protein is a nonsense mutation
False
b. The TIM deficiency mutation changes the tertiary structure of the protein such that the new tertiary structure does not function at high temperatures.
True
c. In the above case, the TIM deficiency does not result from a base substitution
False
a. 5’ UAC AUG CCA UUU GC 3’ (mRNA Sequence)
b. 5’ NH2- TMPF Carboxyl Terminus- COOH- 3’ (Protein Sequence)