Mass of pure acetylsalicylic acid used to prepare the 250 ml pure aspirin stock

Question

Mass of pure acetylsalicylic acid used to prepare the 250 ml pure aspirin stock standard solution and the concentration (mg/mL) of solution. (200.5 mg/250 ml)/1000 L - 0.0008 mg/L times 1000 mL - 0.8020 mg/mL Concentration (mg/mL) and absorbance for each of the four standard acetylsalicylic acid solutions prepared C-1 V_1 = C_1 V_1 0.8020 mg/mL times 10 ml -C(100 ml) C - 0.08 mg/ml volume and absorbance for each of the three "unknown" aspirin tablet solution prepared. Beer's Law plot and equation for the line (calculated using Excel). Y = 6.005x + 0.017 Concentration of each "unknown aspirin tablet solution determined using the Beer's Law plot. Y = 6.005x + 0.017 C = A - B/M = 0.555 - 0.017/6.005 = 0.0896 mg/ml

Explanation / Answer

Determination of aspirin concetration in the unknown sample

1) prepare standard apirin solutions with known concentrations of aspirin in it.

2) measure absorbance of each solution

3) plot, absorbance versus concentration of aspiring in each solution

4) straight line equation from plot,

y = mx + c

with, x = concentration and y = absorbance

5) from solution of unknown sample measure absorbance

6) calculate concentration of aspirin in each unknown sample

The calculations shown above follows thes steps and thus the fial concentration of aspirin in the unknown solution is correct.

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