Ill. Use the DNA sequence below, which encodes a prokaryoticgeneto answer the fo

Question

Ill. Use the DNA sequence below, which encodes a prokaryoticgeneto answer the following questions. ATGAGGAGTT GACACACAAG AGGAGGTAGC AGTATGGGTA TAATCTAATG 51 CGTAATTGAG GAGGTAGTTG ACGTATGAAT AGTTAACGTA CGGGGGGGAA 101 ACCCCCCCTT TTTTTTTTTC GAGCAATAAA AGGGTTACAG ATTGCATGCT 21 71 121 31 81 131 41 91 141 61 a) Write down the corresponding sequences, find them in the sequence above and label them: (label as -35) -35 Consensus sequence: -10 Consensus sequence (Pribnow box):(label as Pribnow) Shine-Dalgarno sequence in corresponding mRNA: Start (initiation) codon in corresponding mRNA: Stop (termination) codon in corresponding mRNA:(label as stop) (label as SD) (label as start) b) What region of this prokaryotic DNA sequence will be transcribed into mRNA? Circle one. 1-131 71-119 74-149 54-119 a) What will the sequence be for the protein translated from this mRNA? c) Where is the 3'UTR? Circle one. 9-131 41-119 81-141 87-119

Explanation / Answer

Answer a) for the 35 consensus sequence from 1-40 base pair:

1 A T G A G G A G T T

11. G A C A C A C A G G

21. A G G A G G T A C C

31. A G T A T G G G T A

For the consensus sequence, as we know TATAAT that binds to promotor or promotor protein. For this also,

Lookong horizontally 1 by 1 line, in 1 st we get 1 guanine and 3 adenine= G/A.

2nd column: 1 Thymine, 1 Adenine and 2 Guanine are present. so, A+T/G

3rd column: G

4th column: A

5th column: G

6th column : G

7th column: A+ T + G + C

8th column: 2A/2G

9th column:G+C/2T

10th column: T+G+C+A

so the final consensus sequence is; G/A A+T/G G A G G

, A+ T+ G+C, 2A/2G, G+C/2T, T + G+ C + A.

-Start codon starts from TATAAT region of 41 bp sequence.

-Stop codon at CAG sequence of 131 bp.

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