The diagram below shows a multiple protein sequence alignment. Rat 1 MARTKQTARKS
ID: 90342 • Letter: T
Question
The diagram below shows a multiple protein sequence alignment. Rat 1 MARTKQTARKSTGGKAPRKQLATKAARPATGGVKHRYRPGTVALREIRRY 50 Horse 1 MARTKQTARKSTGGKAPRKQLATKAARPATGGVKHRYRPGTVALREIRRY 50 Gorilla 1 MARTKQTARKSTGGKAPRKQLATKAARPATGGVKHRYRPGTVALREIRRY 50 Worm 1 MARTKQSARISTOGGKAPKKQLATKGARPAAGGVKHRYR PGTVALREIRRY 50 Fluke 1 MARTKQSARISTGGKAPRKQLATKMARPATGAVKHRYRPGTVALREIRRY 50 Lettuce 1 MARTKQSARISTGGKAPKKQLATKGARPATGAVKHRFRPGTVALREIRKY 50 ******:** ******:****** ****:*.****:***********:* Answer A, B, C, D, and E. How many proteins are being aligned in this analysis? What is the name of a bioinformatics tool that is commonly used to produce this alignment output? What is the percentage identity between the rat and lettuce proteins in this aligned region? Show your calculation. Describe ONE thing that a researcher could learn about the gorilla protein from an alignment of this type? Residue 10 shown in each of the sequences is a K or an I. Is K to I a conservative or non-conservative change? Why? Draw structures of the amino acid side chains and explain their properties as part of your answer.Explanation / Answer
A) Six proteins are aligned in this analysis.
B) The bioinformatics tools that is commonly used to produce this protein alignment are BLAST (Basic Local Alignment Search Tool) and MSA (Multiple Sequence Alignment).
C) There are 50 sequences in both rat and lettuce protein. They match in 43 sequences and differ in 7 sequences. Therefore, the percentage identify between the two protein sequences is 43/50 which is 86%.
D) The percentage identify between the protein sequence of gorilla is very less when compared with the protein sequences of other animals.